Post by account_disabled on Oct 26, 2023 4:54:50 GMT -5
Proteases play physiological roles in humans and when their activity is unregulated they can cause various problems and illnesses. The increased proteolytic activity of elastase, a serine protease produced by the pancreas and neutrophils, is related to some premature aging of the skin and some diseases such as pulmonary emphysema and cystic fibrosis. Considering that plants produce different classes of bioactive compounds, the present study aimed to evaluate the inhibitory effect of crude aqueous and ethanolic extracts of the Cordia Superba plant on the enzymatic activity of elastase.
Both extracts showed relevant inhibition of the proteolytic activity of elastase. The aqueous extract showed a maximum inhibition percentage of 99% at a concentration of 440 µg/ml and IC 50 of 77.3 ± 16.4 µg/ml. The ethanolic extract inhibited with a maximum percentage of 73% at a europe mobile number list concentration of 308 µg/ml. ml, with IC 50 of 154.1 ± 26.5 µg/ml. In conclusion, the results suggest that crude extracts of Cordia Superba can be a good source for prospecting elastase inhibitors. Keywords: Enzymes, elastase, inhibition INTRODUCTION Proteases, proteinases or peptides comprise a group of hydrolase enzymes responsible for catalyzing the hydrolysis reactions of peptide bonds present in proteins or peptides, through selective modifications (WHITAKER, 1994; LOPEZ-OTÍN, 2002; GURUMALLESH et al., 2019 ).
Through proteolytic processing, these enzymes will allow a large number of physiological processes, including: protein digestion, reproduction, blood coagulation, Kallikrein-Kinin system and fibrinolysis (OTÍN and BONDE, 2008). They are also capable of guaranteeing the execution of a series of cellular functions through cascade reactions, among which homeostasis and inflammation stand out (RAO et al., 1998). However, if not controlled, they can generate a series of pathologies in humans, triggering illnesses such as pulmonary emphysema, thrombosis, diabetes, arthritis and cancer (RAWLINGS et al., 2004; DI PAOLO et al., 2019).
Both extracts showed relevant inhibition of the proteolytic activity of elastase. The aqueous extract showed a maximum inhibition percentage of 99% at a concentration of 440 µg/ml and IC 50 of 77.3 ± 16.4 µg/ml. The ethanolic extract inhibited with a maximum percentage of 73% at a europe mobile number list concentration of 308 µg/ml. ml, with IC 50 of 154.1 ± 26.5 µg/ml. In conclusion, the results suggest that crude extracts of Cordia Superba can be a good source for prospecting elastase inhibitors. Keywords: Enzymes, elastase, inhibition INTRODUCTION Proteases, proteinases or peptides comprise a group of hydrolase enzymes responsible for catalyzing the hydrolysis reactions of peptide bonds present in proteins or peptides, through selective modifications (WHITAKER, 1994; LOPEZ-OTÍN, 2002; GURUMALLESH et al., 2019 ).
Through proteolytic processing, these enzymes will allow a large number of physiological processes, including: protein digestion, reproduction, blood coagulation, Kallikrein-Kinin system and fibrinolysis (OTÍN and BONDE, 2008). They are also capable of guaranteeing the execution of a series of cellular functions through cascade reactions, among which homeostasis and inflammation stand out (RAO et al., 1998). However, if not controlled, they can generate a series of pathologies in humans, triggering illnesses such as pulmonary emphysema, thrombosis, diabetes, arthritis and cancer (RAWLINGS et al., 2004; DI PAOLO et al., 2019).